胱天蛋白酶8(CASP8)多克隆抗體
Polyclonal Antibody to Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
- 編號PAA853Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPA853Hu01-胱天蛋白酶8(CASP8)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB,IHC,ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 504¥ 1176¥ 1680¥ 4200¥ 16800
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human CASP8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Human Liver cancer Tissue; <br/>Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Human Lymphoma Tissue<br/>Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colon Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Amygdalitis Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant CASP8, Human.
Western Blot; Sample: Lane1: HepG2 cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: Porcine Spleen lysate; Lane5: Mouse Lung lysate; Lane6: Rat Spleen lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human CASP8 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification: <br/>Lane 1: Wild-type Hela cell lysate; <br/>Lane 2: CASP8 knockout Hela cell lysate; <br/>Predicted MW: 26-32,46,54-62kd <br/>Observed MW: 35kd<br/>Primary Ab: 1μg/ml Rabbit Anti-Human CASP8 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CASP8的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CASP8。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
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| RPA853Hu03 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA853Hu01 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA853Hu02 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; ICC/IF |
| PAA853Hu04 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA853Hu01 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB,IHC,ICC/IF |
| PAA853Hu03 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC/IF |
| FAA853Hu01 | 抗胱天蛋白酶8(CASP8)多克隆抗體 | Flow cytometry. |
| LAA853Hu81 | 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA853Hu71 | 胱天蛋白酶8(CASP8)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA853Hu21 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu23 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu25 | 胱天蛋白酶8(CASP8)單克隆抗體 | IHC |
| MAA853Hu24 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| MAA853Hu29 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| MAA853Hu27 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA853Hu22 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| SEA853Hu | 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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參考文獻
| 雜志 | 參考文獻 |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations[Pubmed:25502462] |
| Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells[Pubmed:29555410] |
| Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway[Pubmed:29266336] |
| Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice[Pubmed: 31087429] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats[Pubmed: 31421311] |
| Journal of Biochemical and Molecular Toxicology | Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation[Pubmed: 32045101] |
| life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats[Pubmed: 32259601] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells[Pubmed: 33206337] |
| Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling[Pubmed: 33199237] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways[Pubmed: 32942000] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?…[Pubmed: 32915453] |
| J Biochem Mol Toxicol | Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation[34047436] |