核仁磷酸蛋白(NPM1)多克隆抗體
Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- 編號PAC664Hu02
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPC664Hu02-核仁磷酸蛋白(NPM1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應用WB,IHC,FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Vector Red staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: Jurkat cell lysate; Lane3: A549 cell lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Human NPM1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Testis lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human U2OS cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human K562 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human NPM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human NPM1 Polyclonal Antibody (Catalog PAC664Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對NPM1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別NPM1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC664Hu01 | 核仁磷酸蛋白(NPM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC664Hu02 | 核仁磷酸蛋白(NPM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC664Hu02 | 核仁磷酸蛋白(NPM1)多克隆抗體 | WB,IHC,FCM |
| PAC664Hu01 | 核仁磷酸蛋白(NPM1)多克隆抗體 | WB,IHC |
| LAC664Hu71 | 核仁磷酸蛋白(NPM1)多克隆抗體(生物素標記) | IHC |
| MAC664Hu22 | 核仁磷酸蛋白(NPM1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC664Hu | 核仁磷酸蛋白(NPM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC664Hu | 核仁磷酸蛋白(NPM1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |