過氧化還原酶4(PRDX4)多克隆抗體
Polyclonal Antibody to Peroxiredoxin 4 (PRDX4)
Antioxidant enzyme AOE372; Thioredoxin-dependent peroxide reductase A0372
- 編號PAF754Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPF754Hu01-過氧化還原酶4(PRDX4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 630¥ 1470¥ 2100¥ 5250¥ 21000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PRDX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Mouse Cerebrum lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Human PRDX4 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: Raji cell lysate; Lane3: K562 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human PRDX4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: Porcine Lung lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human PRDX4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對PRDX4的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PRDX4。
用法
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPF754Hu02 | 過氧化還原酶4(PRDX4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF754Hu01 | 過氧化還原酶4(PRDX4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF754Hu02 | 過氧化還原酶4(PRDX4)多克隆抗體 | WB |
| PAF754Hu01 | 過氧化還原酶4(PRDX4)多克隆抗體 | WB,IHC |
| MAF754Hu21 | 過氧化還原酶4(PRDX4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEF754Hu | 過氧化還原酶4(PRDX4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF754Hu | 過氧化還原酶4(PRDX4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophages[pubmed:27716839] |
| EMBO Journal | Prdx4 limits caspase‐1 activation and restricts inflammasome‐mediated signaling by extracellular vesicles[Pubmed: 31544965] |