癌胚抗原(CEA)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Carcinoembryonic Antigen (CEA) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
（注：單次混測多因子不超過8個指標 ）
- 編號LMA150Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長4h
- 檢測范圍1.22-1250pg/mL
- 靈敏度最小可檢測劑量小于等于0.407 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
- 價格¥ 2090¥ 2171¥ 2291¥ 2452¥ 2613¥ 2854¥ 3216¥ 4020 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測癌胚抗原(CEA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的癌胚抗原(CEA)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-103 | 94 |
| EDTA plasma(n=5) | 78-97 | 86 |
| heparin plasma(n=5) | 95-102 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的癌胚抗原(CEA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中癌胚抗原(CEA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-104% | 89-97% | 78-96% | 79-98% |
| EDTA plasma(n=5) | 79-97% | 86-101% | 78-89% | 93-103% |
| heparin plasma(n=5) | 92-102% | 80-99% | 85-92% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標準品、試劑及樣本準備；
2、加樣（標準品、樣本、磁珠），標準品或樣本100μL及磁珠10μL，25°C酶標板振蕩器孵育2小時；
3、磁吸甩干，加檢測溶液A 100μL，25°C酶標板振蕩器孵育1小時；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將癌胚抗原(CEA)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的癌胚抗原(CEA)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的癌胚抗原(CEA)抗體，加入PE標記的親和素，上機讀數(shù)。MFI值和樣品中的癌胚抗原(CEA)濃度呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA150Hu61 | 癌胚抗原(CEA)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA150Hu61 | 癌胚抗原(CEA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu01 | 癌胚抗原(CEA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu04 | 癌胚抗原(CEA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu03 | 癌胚抗原(CEA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu02 | 癌胚抗原(CEA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA150Hu02 | 癌胚抗原(CEA)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA150Hu04 | 癌胚抗原(CEA)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA150Hu05 | 癌胚抗原(CEA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA150Hu04 | 癌胚抗原(CEA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA150Hu03 | 癌胚抗原(CEA)多克隆抗體 | WB |
| PAA150Hu01 | 癌胚抗原(CEA)多克隆抗體 | WB |
| PAA150Hu02 | 癌胚抗原(CEA)多克隆抗體 | WB |
| LAA150Hu71 | 癌胚抗原(CEA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| RAA150Hu21 | 癌胚抗原(CEA)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA150Hu22 | 癌胚抗原(CEA)單克隆抗體 | Testing in progress |
| SEA150Hu | 癌胚抗原(CEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA150Hu | 癌胚抗原(CEA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA150Hu01 | 癌胚抗原(CEA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model[PubMed: 20872761] |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver[ScienceDirect: S0024320511002700] |
| Iranian journal of basic medical sciences | Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat[PubMed: PMC3637902] |
| Biosensors and Bioelectronics | Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip[Pubmed: 23672876] |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention.[Pubmed:25684529] |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry[Rsc:Source] |
| Breast | Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator[PubMed: 26446295] |
| The Journal of Toxicological Sciences | Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide[PubMed: 26558449] |
| PLoS?One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study[Pubmed:26934615] |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats[2071_pdf.pdf] |
| Analytical chemistry | Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells[pubmed:27506255] |
| Frontiers in Physiology? | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats[v04n07a07a] |
| Analytica?Chimica?Acta | Ultrasensitive cathode photoelectrochemical immunoassay based on TiO2 photoanode-enhanced 3D Cu2O nanowire array photocathode and signal amplification by?…[Pubmed:29866267] |
| Journal of?Cancer | Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung Cancer[Pubmed:29675110] |
| Clinical?Breast?Cancer | 8-Hydroxy-2'-deoxyguanosine as a Discriminatory Biomarker For Early Detection of Breast Cancer[] |
| Journal of Ethnopharmacology | Preventive potential of dietary inclusion of Brachystegia eurycoma (Harms) seeds on N-methyl-N-nitrosourea-induced colon carcinogenesis in Wistar rats[Pubmed: 30953819] |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma[] |
| Biomaterials | DNA-templated porous nanoplatform towards programmed “double-hit” cancer therapy via hyperthermia and immunogenicity activation[Pubmed: 31374481] |
| Biology | Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats[Pubmed: 32967203] |
| Oncology Research and Treatment | Evaluation of the Diagnostic and Predicative Values of 8-Iso-Prostaglandin F2α as a Biomarker of Breast Cancer[Pubmed: 32721979] |
| Journal of Functional Foods | Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in?…[] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles[] | |
| Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways[] | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS[] |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo[] | |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate[] |