促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Corticotropin Releasing Factor (CRF) ,etc. by FLIA (Flow Luminescence Immunoassay)
CRH; Corticotropin Releasing Hormone; Corticoliberin
（注：單次混測多因子不超過8個指標 ）
- 編號LMA835Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長3h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測促腎上皮質(zhì)激素釋放激素(CRF)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的促腎上皮質(zhì)激素釋放激素(CRF)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-98 | 88 |
| EDTA plasma(n=5) | 94-103 | 97 |
| heparin plasma(n=5) | 92-105 | 99 |
| sodium citrate plasma(n=5) | 87-102 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的促腎上皮質(zhì)激素釋放激素(CRF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促腎上皮質(zhì)激素釋放激素(CRF)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 97-104% | 91-98% | 79-90% |
| EDTA plasma(n=5) | 80-102% | 90-101% | 82-96% | 95-102% |
| heparin plasma(n=5) | 86-101% | 79-91% | 91-101% | 92-101% |
| sodium citrate plasma(n=5) | 84-104% | 87-94% | 79-99% | 81-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠、檢測溶液A），標準品或樣本50μL，加檢測溶液A 50μL，及磁珠10μL，25°C酶標板振蕩器孵育90分鐘；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
5、磁吸洗板1次；
6、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將促腎上皮質(zhì)激素釋放激素(CRF)抗體包被于磁性微球表面，制成固相載體。在微孔中同時加入待測抗原（標準品或樣本）、生物素標記的抗原和微球。待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。加入PE標記的鏈霉親和素，上機讀數(shù)。MFI值（Median Fluorescence Intensity）和樣品中的促腎上皮質(zhì)激素釋放激素(CRF)濃度呈負相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA835Hu61 | 促腎上皮質(zhì)激素釋放激素(CRF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA835Hu04 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA835Hu03 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA835Hu01 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA835Hu63 | 促腎上皮質(zhì)激素釋放激素(CRF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA835Hu02 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA835Hu24 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Hu25 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Hu21 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Hu22 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Hu33 | 促腎上皮質(zhì)激素釋放激素(CRF)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA835Hu01 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | IHC |
| PAA835Hu03 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | Testing in progress. |
| PAA835Hu02 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | IHC |
| PAA835Hu04 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB |
| PAA835Hu07 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | Testing in progress. |
| PAA835Hu08 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | Testing in progress. |
| PAA835Hu09 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | Testing in progress. |
| LAA835Hu81 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA835Hu71 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(生物素標記) | WB |
| MAA835Hu22 | 促腎上皮質(zhì)激素釋放激素(CRF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA835Hu21 | 促腎上皮質(zhì)激素釋放激素(CRF)單克隆抗體 | WB |
| CEA835Hu | 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA835Hu | 促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA835Hu11 | 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats[Pubmed:24440345] |
| Bipolar?Disord. | Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?[Pubmed:24862833] |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level[Pubmed:25236411] |
| Ann Dermatol | Acute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth.[pubmed:27746640] |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation[pubmed:28663093] |
| Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation[pubmed:29082242] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats[Pubmed:30028991] |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?…[Pubmed:30038568] |
| Toxicology Letters | Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors[Pubmed:29807116] |
| Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?…[Pubmed:29379286] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?…[Pubmed:29540178] |
| Bioscience Research | Bioscience Research[] |
| FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice[Pubmed: 31431066] |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging[Pubmed: 32463582] |
| Endocr J | Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress[33408312] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress[33651993] |