γ-氨基丁酸(gABA)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay)
4-Aminobutyric Acid
（注：單次混測多因子不超過8個指標 ）
- 編號LMA900Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長3h
- 檢測范圍1.95-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.65 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測γ-氨基丁酸(gABA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的γ-氨基丁酸(gABA)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 82-101 | 97 |
| heparin plasma(n=5) | 86-101 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的γ-氨基丁酸(gABA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中γ-氨基丁酸(gABA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 95-105% | 86-96% | 89-98% |
| EDTA plasma(n=5) | 96-105% | 91-98% | 80-98% | 83-98% |
| heparin plasma(n=5) | 94-105% | 78-101% | 80-91% | 87-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠、檢測溶液A），標準品或樣本50μL，加檢測溶液A 50μL，及磁珠10μL，25°C酶標板振蕩器孵育90分鐘；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
5、磁吸洗板1次；
6、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將γ-氨基丁酸(gABA)抗體包被于磁性微球表面，制成固相載體。在微孔中同時加入待測抗原（標準品或樣本）、生物素標記的抗原和微球。待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。加入PE標記的鏈霉親和素，上機讀數(shù)。MFI值（Median Fluorescence Intensity）和樣品中的γ-氨基丁酸(gABA)濃度呈負相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA900Ge11 | γ-氨基丁酸(gABA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge21 | γ-氨基丁酸(gABA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge41 | γ-氨基丁酸(gABA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA900Ge01 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA |
| PAA900Ge02 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA900Ge71 | γ-氨基丁酸(gABA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA900Ge81 | γ-氨基丁酸(gABA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| CEA900Ge | γ-氨基丁酸(gABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA900Ge | 抗γ-氨基丁酸抗體(Anti-gABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA900Ge | γ-氨基丁酸(gABA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA900Ge11 | γ-氨基丁酸(gABA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology and Industrial Health | Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum[PubMed: 21444355] |
| Neurosci Lett | Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.[PubMed: 18577424] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism[PubMed: PMC3623607] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children[PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)[PubMed: PMC3762604] |
| Scientific Reports | Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension[pubmed:27917928] |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats[Pubmed:28861894] |
| Scientific Reports | A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme[Pubmed: 30341396] |
| Neurochemical?Research | Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission[Pubmed: 30666488] |
| Neuroscience?Letters | Differentiation of human glioblastoma U87 cells into cholinergic neuron[Pubmed: 30928478] |
| Neurochemical Journal | Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism[] |
| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.[] | |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β?hydroxybutyrate on kainic acid?induced epilepsy[Pubmed: 33101467] |
| Exp Anim | Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation[33828024] |
| Neurogenetics | Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases[34101060] |
| Sci Rep | Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission[34475508] |
| J Food Biochem | Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies[Pubmed:35102569] |