沉默調(diào)節(jié)蛋白1(SIRT1)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
（注：單次混測多因子不超過8個指標 ）
- 編號LME912Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長4h
- 檢測范圍0.02-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.007 ng/mL.
- 樣本類型Tissue homogenates, cell lysates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測沉默調(diào)節(jié)蛋白1(SIRT1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的沉默調(diào)節(jié)蛋白1(SIRT1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-99 | 93 |
| EDTA plasma(n=5) | 90-98 | 94 |
| heparin plasma(n=5) | 80-88 | 85 |
| sodium citrate plasma(n=5) | 79-104 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的沉默調(diào)節(jié)蛋白1(SIRT1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中沉默調(diào)節(jié)蛋白1(SIRT1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 86-95% | 89-99% | 95-103% |
| EDTA plasma(n=5) | 81-99% | 93-101% | 95-102% | 81-91% |
| heparin plasma(n=5) | 80-93% | 92-102% | 93-101% | 82-91% |
| sodium citrate plasma(n=5) | 97-105% | 89-104% | 81-97% | 79-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標準品、試劑及樣本準備；
2、加樣（標準品、樣本、磁珠），標準品或樣本100μL及磁珠10μL，25°C酶標板振蕩器孵育2小時；
3、磁吸甩干，加檢測溶液A 100μL，25°C酶標板振蕩器孵育1小時；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將沉默調(diào)節(jié)蛋白1(SIRT1)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的沉默調(diào)節(jié)蛋白1(SIRT1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的沉默調(diào)節(jié)蛋白1(SIRT1)抗體，加入PE標記的親和素，上機讀數(shù)。MFI值和樣品中的沉默調(diào)節(jié)蛋白1(SIRT1)濃度呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPE912Mu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPE912Mu02 | 沉默調(diào)節(jié)蛋白1(SIRT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Mu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體 | WB,ICC/IF |
| PAE912Mu02 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAE912Mu71 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體(生物素標記) | WB |
| SEE912Mu | 沉默調(diào)節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Mu | 沉默調(diào)節(jié)蛋白1(SIRT1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSE912Mu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
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| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats[Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment[Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma[PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:[PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.[pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury[pubmed:28361247] |
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| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial[pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population[pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)[pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction[pubmed:28919305] |
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| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors[Pubmed: 30952517] |
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| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor[Pubmed: 33045669] |
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| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction[] |
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| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??-[33396247] |
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| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ?-[34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories[34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats[34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease[34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats[Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?[Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress[] |