聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Aggrecan (AGC) ,etc. by CBA (Cytometric Bead Array)
ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-98 | 90 |
| EDTA plasma(n=5) | 81-98 | 89 |
| heparin plasma(n=5) | 79-90 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-102% | 89-96% | 78-104% | 78-97% |
| EDTA plasma(n=5) | 97-105% | 87-101% | 79-103% | 86-102% |
| heparin plasma(n=5) | 87-99% | 78-93% | 83-98% | 79-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
3、加樣（標(biāo)準(zhǔn)品、樣本、磁珠），標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，25°C酶標(biāo)板振蕩器孵育2小時；
4、磁吸甩干，加檢測溶液A 100μL，25°C酶標(biāo)板振蕩器孵育1小時；
5、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)抗體，加入PE標(biāo)記的親和素，用流式細胞儀讀數(shù)。MFI值和樣品中的聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法)濃度呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB908Rb01 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB908Rb51 | 聚集蛋白聚糖(AGC)多克隆抗體 | Testing in progress |
| MAB908Rb21 | 聚集蛋白聚糖(AGC)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB908Rb | 聚集蛋白聚糖(AGC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB908Rb | 聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| CMB908Rb | 聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells[Rsmjournals: Source] |
| Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency[Springer: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.[Pubmed: 24739658] |
| Materials Science and Engineering: C | Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition[ScienceDirect: S0928493113006656] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| Macromolecular Bioscience | Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration[PubMed: 25787871] |
| Osteoarthritis and Cartilage | Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs[Pubmed:26802547] |
| Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes[Pubmed:27090063] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731] |
| Journal of Clinical Medicine | Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors[Pubmed: 32392807] |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[] | |
| researchsquare | Aggrecan-a new biomarker for acute type A aortic dissection[33990642] |