饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array)
MTLRP; Growth Hormone-Releasing Peptide
- 編號CMA991Ov
- 物種Ovis aries; Ovine (Sheep，綿羊)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長3h
- 檢測范圍9.7656-10000pg/mL
- 靈敏度最小可檢測劑量小于等于3.2552pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T
- 價格¥ 4082¥ 5832
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 89-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-99% | 82-97% | 87-95% | 83-101% |
| EDTA plasma(n=5) | 87-95% | 79-95% | 94-102% | 78-91% |
| heparin plasma(n=5) | 79-102% | 89-96% | 79-97% | 95-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
3、加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A），標(biāo)準(zhǔn)品或樣本50μL，加檢測溶液A 50μL，及磁珠10μL，25°C酶標(biāo)板振蕩器孵育90分鐘；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
5、磁吸洗板1次；
6、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體。在微孔中同時加入待測抗原（標(biāo)準(zhǔn)品或樣本）、生物素標(biāo)記的抗原和微球。待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。加入PE標(biāo)記的鏈霉親和素，上機讀數(shù)。MFI值（Median Fluorescence Intensity）和樣品中的饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法)濃度呈負相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Ovis aries; Ovine (Sheep，綿羊) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA991Ov | 饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMA991Ov | 饑餓素(GHRL)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
| LMA991Ov | 饑餓素(GHRL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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