谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Glutathione Peroxidase 1 (GPX1) ,etc. by CBA (Cytometric Bead Array)
GSHPX1; Cellular glutathione peroxidase
產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-102 | 94 |
| EDTA plasma(n=5) | 88-102 | 96 |
| heparin plasma(n=5) | 91-101 | 96 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 88-95% | 85-99% | 93-105% |
| EDTA plasma(n=5) | 85-101% | 86-99% | 97-105% | 78-101% |
| heparin plasma(n=5) | 90-102% | 97-104% | 83-94% | 87-96% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向實驗板每孔內加入200μL分析緩沖液以預濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠），標準品或樣本100μL及磁珠10μL，25°C酶標板振蕩器孵育2小時；
4、磁吸甩干，加檢測溶液A 100μL，25°C酶標板振蕩器孵育1小時；
5、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數。
實驗原理
將谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)與連接于固相載體上的抗體結合，然后加入生物素化的谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)抗體，加入PE標記的親和素，用流式細胞儀讀數。MFI值和樣品中的谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法)濃度呈正相關。
贈品
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA295Bo01 | 谷胱甘肽過氧化酶1(GPX1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA295Bo01 | 谷胱甘肽過氧化酶1(GPX1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA295Bo21 | 谷胱甘肽過氧化酶1(GPX1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA295Bo | 谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA295Bo | 谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| CMA295Bo | 谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One. | P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation[Plos:Source] |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure[Pubmed:24443098] |
| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer[Pubmed:25550558] |
| Adv Med Sci | Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, [PubMed: 26674569] |
| Applied Biological Research | Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditions[publication:301246301] |
| Cell Biol Int | Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions[fileID:697741] |
| Toxicology Mechanisms and Methods | Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism[pubmed:27599793] |
| Journal of Environmental Research and Public Health | Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas.[pubmed:28574428] |
| Pharmacological Reports | Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder.[pubmed:28958613] |
| CNS Spectrums | Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study[pubmed:29248027] |
| Environmental toxicology and pharmacology | Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress[pubmed:28654832] |
| REVISTA DE CHIMIE | Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditis[pdf:44%20PREDA%20C%207%2017.pdf] |
| Biological Rhythm Research? | Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate[10.1080:09291016.2017.1361584] |
| Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer[] | |
| Kolon Kanserinde Gpx1 Gen Anlat?m?, Serum Gpx1 ve Selenyum Düzeylerinin ?ncelenmesi[] | |
| Alcohol. | Influence of deficient nutrition on trace element status and antioxidant defense system[Pubmed: 1781925] |
| BMC Infectious Diseases | Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching?…[Pubmed: 31703562] |
| LIFE SCIENCES | Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway[Pubmed: 32798557] |
| AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS[] | |
| The Journal of Physiology | In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells[] |
| World Neurosurg | Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ?-[33689849] |
| J Pers Med | Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis[34945751] |
| J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease[Pubmed:35086715] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating?…[Pubmed:35281603] |