凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) ,etc. by CBA (Cytometric Bead Array)
OLR1; CLEC8A; SCARE1; Oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-89 | 82 |
| EDTA plasma(n=5) | 85-95 | 91 |
| heparin plasma(n=5) | 82-96 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-93% | 84-91% | 87-99% | 88-95% |
| EDTA plasma(n=5) | 81-94% | 87-95% | 81-97% | 97-105% |
| heparin plasma(n=5) | 83-104% | 94-104% | 81-90% | 89-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠），標準品或樣本100μL及磁珠10μL，25°C酶標板振蕩器孵育2小時；
4、磁吸甩干，加檢測溶液A 100μL，25°C酶標板振蕩器孵育1小時；
5、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)抗體，加入PE標記的親和素，用流式細胞儀讀數(shù)。MFI值和樣品中的凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法)濃度呈正相關(guān)。
贈品
增值服務(wù)
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| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB859Ra01 | 凝集素樣氧化低密度脂蛋白受體1(LOX1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB859Ra01 | 凝集素樣氧化低密度脂蛋白受體1(LOX1)多克隆抗體 | WB; IHC |
| MAB859Ra21 | 凝集素樣氧化低密度脂蛋白受體1(LOX1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB859Ra | 凝集素樣氧化低密度脂蛋白受體1(LOX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMB859Ra | 凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
| LMB859Ra | 凝集素樣氧化低密度脂蛋白受體1(LOX1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Clinical Cardiology | Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels Are Associated With Angiographic Coronary Lesion Complexity in Patients With Coronary Artery Diseas[PubMed: 21400544] |
| Coronary Artery Disease | Postprocedural serum sLOX-1 levels are associated with coronary in-stent restenosis in patients with stable coronary artery disease[LWW: 06000] |
| Clinical Biochemistry | Soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in synovial fluid are correlated with disease severity of knee osteoarthritis[ScienceDirect: S0009912011004905] |
| Mediators of Inflammation | Pregnancy Followed by Delivery May Affect Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels in Women of Reproductive Age[PubMed: 22619487] |
| Dicle Medical Journal | Lectin-like oxidized low density lipoprotein receptor 1(LOX-1) levels and endothelial dysfunction in patients with primary essential hyperhidrosis/Primer esansiyel hiperhidroz hastalarinda lektin benzeri okside düsük dansiteli lipoprotein reseptor 1(LOX-1) düzeyleri ve endotel disfonksiyonu[Readperiodicals: Source] |
| Journal of Clinical Laboratory Analysis | Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome[Pubmed:24798146] |
| Journal of Human Hypertension | Endothelial damage in white coat hypertension: role of lectin-like oxidized low-density lipoprotein-1[Pubmed:25007999] |
| Gynecologic and Obstetric Investigation | Inhibition of Lectin-Like Oxidized Low-Density Lipoprotein Receptor 1 Protects against Plasma/Hypoxia-Mediated Trophoblast Dysfunction Associated with Preeclampsia[Pubmed:25633310] |
| World J Gastroenterol | Increased serum soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in patients with biopsy-proven nonalcoholic fatty liver disease[PubMed: 26185381] |
| Genetic Testing and Molecular Biomarkers | Serum sLOX-1 Levels Are Correlated with the Presence and Severity of Obstructive Sleep Apnea[PubMed: 25825846] |
| Neurotoxicology. | 27-Hydroxycholesterol regulates cholesterol synthesis and transport in C6 glioma cells.[pubmed:28167099] |
| Journal of the American?Heart?Association | Increased Levels of Lectin‐Like Oxidized Low‐Density Lipoprotein Receptor‐1 in Ischemic Stroke and Transient Ischemic Attack[Pubmed:29330254] |
| Higher serum lectin-like oxidized low-density lipoprotein receptor-1 in patients with stable coronary artery disease is associated with major adverse?…[] | |
| Disease Markers | Baseline Serum sLOX-1 Concentrations Are Associated with 2-Year Major Adverse Cardiovascular and Cerebrovascular Events in Patients after Percutaneous?…[Pubmed: 31772687] |
| Reproductive Biology | Polycystic ovary syndrome and endothelial dysfunction: A potential role for soluble lectin-like oxidized low density lipoprotein receptor-1[Pubmed: 32409108] |
| Int J Cancer | The oxidized‐LDL/LOX‐1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells[Pubmed:35608341] |