神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array)
NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY
- 編號CMA879Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長3h
- 檢測范圍0.1953-200pg/mL
- 靈敏度最小可檢測劑量小于等于0.0651pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T
- 價格¥ 3268¥ 4668
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-99 | 96 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 80-96 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 84-97% | 90-101% | 92-101% |
| EDTA plasma(n=5) | 80-98% | 78-91% | 93-101% | 84-104% |
| heparin plasma(n=5) | 78-90% | 80-97% | 78-90% | 86-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠、檢測溶液A），標準品或樣本50μL，加檢測溶液A 50μL，及磁珠10μL，25°C酶標板振蕩器孵育90分鐘；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
5、磁吸洗板1次；
6、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體。在微孔中同時加入待測抗原（標準品或樣本）、生物素標記的抗原和微球。待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。加入PE標記的鏈霉親和素，上機讀數(shù)。MFI值（Median Fluorescence Intensity）和樣品中的神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法)濃度呈負相關(guān)。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA879Mu02 | 神經(jīng)肽Y(NPY)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA879Mu01 | 神經(jīng)肽Y(NPY)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA879Mu21 | 神經(jīng)肽Y(NPY)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA879Mu02 | 神經(jīng)肽Y(NPY)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA879Mu01 | 神經(jīng)肽Y(NPY)多克隆抗體 | WB; IHC; ICC; IP. |
| MEA879Mu | 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA879Mu | 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA879Mu | 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMA879Mu | 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
| LMA879Mu | 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA879Mu11 | 神經(jīng)肽Y(NPY)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Toxicology Letters | Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate[Pubmed:25623392] |
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| Journal of Diabetes Research | High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate …[Pubmed:26885531] |
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| basic & clinical pharmacology & toxicology | Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells.[pubmed:27625336] |
| Acta?Neurobiologiae Experimentalis | Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat[pubmed:27685769] |
| Cardiovascular?research | Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis[Pubmed:29850786] |
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| Neuropeptides | Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor[Pubmed:29685637] |
| Pharmacology | Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y?…[Pubmed: 30227410] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome[] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.[] |
| neurochemical research | Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats.[Pubmed: 32378074] |
| Hum Psychopharmacol | Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double?\blind randomized??-[33245168] |
| Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo[34148100] |