酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Tyrosine Hydroxylase (TH) ,etc. by CBA (Cytometric Bead Array)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒對檢測酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)具有較高的靈敏度。
經(jīng)檢測人、小鼠、大鼠之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-88 | 85 |
| EDTA plasma(n=5) | 88-102 | 95 |
| heparin plasma(n=5) | 91-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 87-95% | 92-105% | 90-104% |
| EDTA plasma(n=5) | 89-96% | 85-102% | 78-92% | 87-98% |
| heparin plasma(n=5) | 96-103% | 90-105% | 88-96% | 82-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1、向?qū)嶒灠迕靠變?nèi)加入200μL分析緩沖液以預(yù)濕；
2、實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
3、加樣（標(biāo)準(zhǔn)品、樣本、磁珠），標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，25°C酶標(biāo)板振蕩器孵育2小時；
4、磁吸甩干，加檢測溶液A 100μL，25°C酶標(biāo)板振蕩器孵育1小時；
5、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)抗體，加入PE標(biāo)記的親和素，用流式細(xì)胞儀讀數(shù)。MFI值和樣品中的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法)濃度呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SEB438Mi | 酪氨酸羥化酶(TH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMB438Mi | 酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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